jarid2 d6m9x  (Cell Signaling Technology Inc)

Journal: Molecular cell

Article Title: Unbalanced chromatin binding of Polycomb complexes drives neurodevelopmental disorders

doi: 10.1016/j.molcel.2026.01.023

Figure Lengend Snippet: (A) Strategy to generate Rnf2 WT/R70H ESCs by homologous recombination. (B) DEG from WT and two clones of Rnf2 WT/R70H ESCs (log 2 fold > 2, q < 0.01). n = 2 independent experimental replicates. (C) GO of upregulated genes in Rnf2 WT/R70H ESCs. (D) Heatmaps of Ring1b, H3K27me3, and H2AK119ub ChIP-seq (average signal of two independent experimental replicates) in WT and clone #1 of Rnf2 WT/R70H ESCs. (E) Strategy to generate HA and FLAG-tagged Rnf2 alleles by CRISPR-Cas9 in WT and Rnf2 WT/R70H ESCs. (F) Normalized Ring1b WT and Ring1b R70H Cut&Run signals in WT and Rnf2 WT/R70H ESCs. Signal was generated from two biological replicates from two independent WT and Rnf2 WT/R70H clones. HA and FLAG Cut&Run signals were merged (average of 4 replicates) to avoid potential bias from the HA and FLAG antibodies’ efficiency. (G) Anti-FLAG IPs in Rnf2 HA-WT/FLAG-R70H and Rnf2 FLAG-WT/HA-R70H ESCs followed by LC-MS/MS in three independent experimental replicates. Results are normalized to IgG as a negative control. Volcano plot shows proteins enriched or weakened in FLAG-Ring1b R70H compared with FLAG-Ring1b WT from Rnf2 WT/R70H ESCs. (H) Heatmaps of Cbx7 and Pcgf2, Rybp, Mtf2/Pcl2, and Jarid2 ChIP-seq (average signal of two independent experimental replicates) in WT and clone #1 of Rnf2 WT/R70H ESCs. (I) Genome browser screenshots of ChIP-seq from (H). (J) Mutabind2 scores upon the human RING1B R70H variant vs. full length and lacking their IDR, PCGF1-6 using AlphaFold and ColabFold. (K) Full-length Pcgf2 or lacking the IDR used in (L). (L) Anti-HA IPs followed by WBs against HA, Phc1, and Ring1b in WT and Rnf2 WT/R70H ESCs expressing HA-Pcgf2 WT or HA-Pcgf2 ΔIDR . (M) Model of PRC1/2 recruitment in Rnf2 WT/R70H ESCs. See also .

Article Snippet: JARID2 (D6M9X) (ChIP-seq) , Cell Signaling Technology , Cat# 13594, RRID:AB_2798269.

Techniques: Homologous Recombination, Clone Assay, ChIP-sequencing, CRISPR, Generated, Liquid Chromatography with Mass Spectroscopy, Negative Control, Variant Assay, Expressing

Journal: Molecular Cell

Article Title: PRC2.1- and PRC2.2-specific accessory proteins drive recruitment of different forms of canonical PRC1

doi: 10.1016/j.molcel.2023.03.018

Figure Lengend Snippet:

Article Snippet: JARID2; ChIP, WB , Cell Signaling , Cat#13594 (D6M9X); RRID: AB_2798269.

Techniques: Recombinant, Mutagenesis, Isolation, Western Blot, Software

Journal: Molecular Cell

Article Title: BAP1 enhances Polycomb repression by counteracting widespread H2AK119ub1 deposition and chromatin condensation

doi: 10.1016/j.molcel.2021.06.020

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-JARID2 (D6M9X) , Cell Signaling , Cat #13594; RRID: AB_2798269.

Techniques: Control, Virus, Recombinant, Transfection, Purification, Extraction, Sequencing, Imaging, Plasmid Preparation, Software

Journal: Molecular cell

Article Title: Unique Structural Platforms of Suz12 Dictate Distinct Classes of PRC2 for Chromatin Binding

doi: 10.1016/j.molcel.2018.01.039

Figure Lengend Snippet: (A) Domain architecture of the Jarid2- and Aebp2-containing holo-PRC2 complex is outlined. Compositions of the catalytic module and nucleosome-binding module of the core PRC2 complex are indicated. Protein domains of Suz12, Rbbp4, Jarid2 and Aebp2 that are included in the crystal structure of the heterotetrameric complex are color coded and labeled. Domains not included in the structure are in gray.

Article Snippet: JARID2 (D6M9X) Rabbit mAb , Cell Signaling , Cat# 13594T.

Techniques: Binding Assay, Labeling

Journal: Molecular cell

Article Title: Unique Structural Platforms of Suz12 Dictate Distinct Classes of PRC2 for Chromatin Binding

doi: 10.1016/j.molcel.2018.01.039

Figure Lengend Snippet: Data collection and refinement statistics.

Article Snippet: JARID2 (D6M9X) Rabbit mAb , Cell Signaling , Cat# 13594T.

Techniques:

Journal: Molecular cell

Article Title: Unique Structural Platforms of Suz12 Dictate Distinct Classes of PRC2 for Chromatin Binding

doi: 10.1016/j.molcel.2018.01.039

Figure Lengend Snippet: (A) Surface representation of the composite Suz12 surface formed by the Zn (violet) and ZnB (cyan) domains. The TR domain of Jarid2 is shown in cartoon with a limon color. The rotation matrix relative to the Top view of the heterotetrameric complex in Fig. 1B is provided.

Article Snippet: JARID2 (D6M9X) Rabbit mAb , Cell Signaling , Cat# 13594T.

Techniques:

Journal: Molecular cell

Article Title: Unique Structural Platforms of Suz12 Dictate Distinct Classes of PRC2 for Chromatin Binding

doi: 10.1016/j.molcel.2018.01.039

Figure Lengend Snippet: (A) The structure of Suz12 is shown based on Fig. S4. The binding partners of the each domain of Suz12 in the Jarid2 and Aebp2-containing 6-subunit PRC2 holo complex are indicated.

Article Snippet: JARID2 (D6M9X) Rabbit mAb , Cell Signaling , Cat# 13594T.

Techniques: Binding Assay

Journal: Molecular cell

Article Title: Unique Structural Platforms of Suz12 Dictate Distinct Classes of PRC2 for Chromatin Binding

doi: 10.1016/j.molcel.2018.01.039

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: JARID2 (D6M9X) Rabbit mAb , Cell Signaling , Cat# 13594T.

Techniques: Produced, Recombinant, Suspension, Avidin-Biotin Assay, Labeling, Software